The present work was performed to evaluate the incidence of gene mutation affecting
factor V Leiden (FVL) &factor XIII in cases of myocardial ischemia. Thirty cases of
myocardial ischemia as well as ten age and sex matched healthy subjects were
included in the study. DNA was extracted from blood samples drawn from all subjects
included in the study and was subjected to PCR amplification for factors V (Leiden)
and factor XIII genes, using biotinylated specific primers. The PCR products were
hybridized using strip containing immobilized allele-specific oligonucliotide probe.
The products of hybridization were visualized using striptavidin-alkaline pH and
color substrate. LDL-c was significantly higher in heterozygous factor V leiden The
results of the study showed that heterozygous mutations involving factor V Leiden
cases were in a positive correlation with LDL-c results and homozygous mutations
involving factor XIII cases showed a positive correlation with AST changes. These
results gave an idea about the mutational changes involving factors V Leiden and
XIII and may be considered as risk factors for the incidence of myocardial infarction.