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34412

Effect of testosterone on the cereble cortex Bcl-2 expression and oxidative stress in a rat model of vascular dementia

Article

Last updated: 03 Jan 2025

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-

Tags

Neurophysiology

Abstract

Objectives to investigate the effect of testosterone on the cerebral cortex Bcl-2 expression and oxidative stress in a rat model of vascular dementia (VD). Methods Forty-eight Sprague-Dawley adult male rats were divided into four groups (n=12, each) as follows (i) untreated control, (ii) rats exposed to surgical permanent bilateral occlusion of the common carotid arteries (BCCAO) leading to chronic cerebral hypoperfusion, (iii) rats exposed to BCCAO then received testosterone (0.5 mg/ kg S.C., three times/ week, S.C.) (BCCAO + Tes) for 4 weeks and (iv) rats exposed to BCCAO then received donepezil (3 mg/kg/ day; i.p.) (BCCAO + DON) for 4 weeks. Levels of acetylcholine and norepinephrine in the cerebral cortex were measured. Furthermore, the expression of Bcl-2 in the cerebral cortex and malondialdehyde (MDA), non-enzymatic (reduced glutathione, GSH) and enzymatic [superoxide dismutase (SOD) and catalase (CAT)] anti-oxidants were determined. Histopathological studies of the cerebral cortex were performed. Results BCCAO decreased the expression of Bcl-2 as well as the central level of acetylcholine and norepinephrine as compared to control rats. Also, increased the cerebral cortex level of MDA and decreased the enzymatic and non-enzymatic antioxidant. Treatment with testosterone and donepezil increased acetylcholine and norepinephrine significantly when compared with BCCAO rats. Testosterone significantly increased the expression of Bcl-2 and antioxidant system with significant reduction in MDA. Conclusion Testosterone might employ neuroprotective effects through increasing the expression of Bcl-2 as well as ameliorating the oxidative stress induced by BCCAO in rat model of DA.

DOI

10.21608/besps.2014.34412

Keywords

Acetylcholine, Bcl-2, Donepezil, testosterone, Vascular dementia, Oxidative Stress

Authors

First Name

Hussein

Last Name

Sakr

MiddleName

-

Affiliation

Medical Physiology Department1, Mansoura Faculty of Medicine, Egypt, Medical Physiology Department2, College of Medicine, King Khalid University, KSA

Email

sakr_doctor@yahoo.com

City

-

Orcid

-

First Name

Abd El-Aziz

Last Name

Hussein

MiddleName

-

Affiliation

Medical Physiology Department, Mansoura Faculty of Medicine, Egypt,

Email

-

City

-

Orcid

-

First Name

Khaled

Last Name

Khalil

MiddleName

-

Affiliation

Medical Physiology Department, Mansoura Faculty of Medicine, Egypt,

Email

-

City

-

Orcid

-

First Name

Amr

Last Name

Abbas

MiddleName

-

Affiliation

Medical Physiology Department, Mansoura Faculty of Medicine, Egypt,

Email

-

City

-

Orcid

-

Volume

34

Article Issue

1

Related Issue

5686

Issue Date

2014-06-01

Receive Date

2014-04-10

Publish Date

2014-06-01

Page Start

88

Page End

103

Print ISSN

1110-0842

Online ISSN

2356-9514

Article File

BESPS_Volume 34_Issue 1_Pages 88-103.pdf

PDF . 708.4kB

Link

https://besps.journals.ekb.eg/article_34412.html

Detail API

https://besps.journals.ekb.eg/service?article_code=34412

Order

7

Type

Original Article

Type Code

567

Publication Type

Journal

Publication Title

Bulletin of Egyptian Society for Physiological Sciences

Publication Link

https://besps.journals.ekb.eg/

MainTitle

Effect of testosterone on the cereble cortex Bcl-2 expression and oxidative stress in a rat model of vascular dementia

Details

Type

Article

Created At

22 Jan 2023