ManageChatDiscover
34019

Effects of co-application of corticosterone and growth hormone on hippocampal neurons involve nmda receptor upregulation of nr2b protein expression and increasing nr2b/nr2a ratio

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Neurophysiology

Abstract

Objectives to investigate the possible mechanism underlying the protective effect of growth hormone (GH) on hippocampal function during periods of acute stress. Methods the effects of co-application of GH and corticosterone (CORT) at different concentration on field excitatory postsynaptic potential (fFEPSPs) of hippocampal slices of rats at two different age groups were examined. Also, the protein expression of N-methyl-D-aspartate receptor (NMDARs) subunits; NR1, NR2B, and NR2A in hippocampal brain slices treated with artificial cerebrospinal fluid (ACSF) or low concentration of CORT alone or both CORT and GH for three hours were measured. Results We found an additive effect of co-application of CORT and GH on hippocampal synaptic transmission compared to CORT alone. Furthermore, we found that the combined use of low concentration of GH and CORT have significantly higher effects on enhancement of fFEPSPs in old rats compared to young ones. We showed that both GH and CORT enhanced protein expression of NR2A subunit of NMDARs. Meanwhile, we demonstrated that the coexposure to low concentration of GH and CORT significantly enhanced NR2B expression and increased the NR2B/NR2A. In contrast, perfusion with CORT alone caused significant suppression in NR1 and NR2B protein expression and decrease in NR2B/NR2A. Conclusion we suggest that NMDARs provide potential target for mediating GH potential protective effect against stress and age related memory and cognitive impairment

DOI

10.21608/besps.2014.34019

Keywords

Growth hormone (GH), Corticosterone (CORT), Stress, Field excitatory postsynaptic potentials (FEPSPs), N-methyl-D-aspartate receptors (NMDARs), Hippocampus, Synaptic plasticity

Authors

First Name

Ghada

Last Name

Mahmoud

MiddleName

-

Affiliation

Department of Medical Physiology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Email

-

City

-

Orcid

-

First Name

Ayman

Last Name

Amer

MiddleName

-

Affiliation

Department of Human Anatomy and Embryology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Email

-

City

-

Orcid

-

Volume

34

Article Issue

1

Related Issue

5686

Issue Date

2014-06-01

Receive Date

2014-02-10

Publish Date

2014-06-01

Page Start

11

Page End

26

Print ISSN

1110-0842

Online ISSN

2356-9514

Article File

BESPS_Volume 34_Issue 1_Pages 11-26.pdf

PDF . 867.5kB

Link

https://besps.journals.ekb.eg/article_34019.html

Detail API

https://besps.journals.ekb.eg/service?article_code=34019

Order

2

Type

Original Article

Type Code

567

Publication Type

Journal

Publication Title

Bulletin of Egyptian Society for Physiological Sciences

Publication Link

https://besps.journals.ekb.eg/

MainTitle

Effects of co-application of corticosterone and growth hormone on hippocampal neurons involve nmda receptor upregulation of nr2b protein expression and increasing nr2b/nr2a ratio

Details

Type

Article

Created At

22 Jan 2023