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8644

MicroRNA expression in chronic Hepatitis C patients treated with combined pegylated interferon alpha and ribavirin therapy

Article

Last updated: 03 Jan 2025

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-

Tags

GIT Physiology

Abstract

The role of microRNAs (miRNA) in many diseases, including chronic hepatitis, represents an interesting research field. The current work aimed to study the effect of combination therapy (pegylated interferon alpha-2b plus ribavirin) on microRNA-128 (miR-128) and microRNA-296 (miR-296-5p) expression in patients with chronic hepatitis C. Also, we aimed to investigate the potential value of both types of microRNAs in differentiation between responders and non-responders either in the pre-treatment or post-treatment groups. Sixteen patients with chronic hepatitis C were compared to 10 healthy, age and sex matched controls. Venous blood samples were withdrawn before initiation of combination therapy and 12 weeks later and qPCR was used for gene expression assays. Comparison of pre-treatment group to the control group showed significant miR-128 down-regulation, while miR-296-5p showed a non-significant downregulation. Regarding post-treatment group, there was non-significant upregulation of miR-128, while miR-296-5p was significantly downregulated compared to either pre-treatment group or the control group. Comparative studies of miR-128 and miR-296-5p in responders versus non-responders showed non-significant fold change either in the pre- or post-treatment groups. It could be concluded that chronic hepatitis C as well as the combination therapy modulate miR-128/296 expression. Until now, miRNAs studied were not useful indicators to differentiate between responders and non-responders to combination therapy either in pre- or post-treatment group.

DOI

10.21608/besps.2016.8644

Keywords

miRNA, Chronic HCV, Combination therapy

Authors

First Name

Awad

Last Name

El-abd

MiddleName

-

Affiliation

Departments of Medical Biochemistry,and Biochemical Endocrinology Unit, Faculty of Medicine, Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

Magdi

Last Name

El-sadek

MiddleName

-

Affiliation

Hepatology, Gastroenterology and Infectious Diseases,Faculty of Medicine, Benha University, Egypt.

Email

-

City

-

Orcid

-

First Name

Inas

Last Name

Ahmed

MiddleName

A.

Affiliation

Department of Medical Biochemistry, Molecular Biology and Biotechnology Unit, Faculty of Medicine, Benha University, Al Qalyubia, Egypt

Email

inas.ahmed@fmed.bu.edu.eg

City

-

Orcid

-

First Name

Shuzan

Last Name

Mohammed

MiddleName

Ali

Affiliation

Department of Medical Biochemistry, Molecular Biology and Biotechnology Unit, Faculty of Medicine, Benha University, Al Qalyubia, Egypt.

Email

-

City

-

Orcid

-

First Name

Hend

Last Name

El-sayed

MiddleName

-

Affiliation

Departments of Medical Biochemistry,Faculty of Medicine, Benha University, Egypt.

Email

-

City

-

Orcid

-

Volume

36

Article Issue

1

Related Issue

1655

Issue Date

2016-06-01

Receive Date

2018-07-04

Publish Date

2016-06-01

Page Start

42

Page End

52

Print ISSN

1110-0842

Online ISSN

2356-9514

Link

https://besps.journals.ekb.eg/article_8644.html

Detail API

https://besps.journals.ekb.eg/service?article_code=8644

Order

5

Type

Original Article

Type Code

567

Publication Type

Journal

Publication Title

Bulletin of Egyptian Society for Physiological Sciences

Publication Link

https://besps.journals.ekb.eg/

MainTitle

MicroRNA expression in chronic Hepatitis C patients treated with combined pegylated interferon alpha and ribavirin therapy

Details

Type

Article

Created At

22 Jan 2023