This study was carried out during 2020 and 2021 seasons to examine the effect of spraying glutathione at 0.025 to 0.1% and/ or citrine compound (2% Zn + 2% Fe + 2% Mn) at 0.05 to 0.2 % were sprayed three times from growth start (first week of March), just after berry setting (first week of Apr.) and three weeks later (last week of Apr.). on growth aspects, vine nutritional status, yield , berries colouration % as well as physical and chemical characteristics of Flame seedless grapes, Subjecting the vines to glutathione at 0.025 to 0.1% and or citrine compound at 0.05 to 0.2% was very effective in enhancing main shoot length, number of leaves/ shoot, leaf area, wood ripening coefficient, cane thickness, pruning wood weight, as well as leaf photosynthesis pigments, N, p, K , Fe, Zn and Mn in the leaves , yield, cluster weight and dimensions, berries colouration % and both physical and chemical characteristics compared to the control treatment. Using citrine compound was favourable than using glutathione in this respect Combined application of glutathione and citrine compound obviously surpassed the application of each alone in this respect, The best results with regard to yield berries colouration % and quality of the berries were recorded on the vines that received three sprays (at growth start, just after berry setting and three weeks later) of glutathione at 0.05% and citrine compound (2% Zn + 2% Fe + 2% Mn) at 0.1%.