In vitro experiment was conducted at the Plant Tissue Culture Laboratory of the Department of
Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt during the period 2015-2016. Effects of
increasing number of subcultures up to 12th subculture, BA concentrations up to 2.0 mg/l and their interaction on
multiplication rate of four strawberry cultivars were studied. Also, organogenic potential of leaf disc explants from
three strawberry cultivars were tested using two different media (direct and indirect regeneration medium). Presented
results showed that the highest multiplication rate in cvs. Kamela and Amega were achieved after the 6th subcultures
using 1.0 mg/l BA, however, the highest multiplication rate in cv. Camarosa was achieved after 7 subcultures using 1.0
mg/ BA. The highest multiplication rate in cv. Fortuna was found after 6 subcultures on MS medium+0.5 mg/l BA,
however, the lowest multiplication rate in all tested genotypes were after 12th subculture on MS+ 2.0 mg/l BA. High
regeneration capacity was obtained in medium amended with TDZ (direct regeneration medium) compared with 2,4-
D+BA (indirect regeneration medium). Fortuna gave the highest regenerated shoots followed by cv. Kamela, then cv.
Camarosa (v/v/v). Plantlets were successfully acclimated with 90% survival.