This study was carried out at the Plant Tissue Culture Laboratory in Faculty of Environmental
Agricultural Sciences (FEAS), Arish University, Egypt during 2012 through 2015. The aim of this study was using
tissue culture technique for micropropagation of peach (Prunus persica L.) plant. Nodes and shoot tips were cultured on
MS, NN, WPM and B5 media containing macro and micro elements as well as vitamins, supplemented with 100 mgl-
1myo-inositol and 30 % sucrose. Shoot tip cultured on the MS medium had the best combination for the establishment
stage of mother plants. Multiple shoot tips were obtained on MS medium supplemented with 1.00 mgl-1 BA and 0.05
mgl-1 NAA. The highest elongation of shoots was with 1.0 mgl-1 GA3.The highest rooting of shoots was with full
strength MS medium with 2.00 mgl-1 IBA. Hardening the rooted shoots was done in a greenhouse in pots containing
mixture of peatmoss, vermiculite and sand at the rate1:1:1. Plantlets were successfully acclimated with 93 % survive