This study was carried out at the Plant Tissue Culture Laboratory, Faculty of Environmental Agricultural
Sciences (FEAS), Arish University, North Sinai, Egypt during 2012 to 2017. The aim of the study was to use tissue culture technique for the micropropagation of Marrubium vulgare plant. One node cutting and shoot tip were cultured on MS, NN and B5 media supplemented with 100 mgl-1 myo-inositol and 3% sucrose. The results showed that shoot tip cultured on the MS medium had the best effect on the establishment stage of mother plants. Multiple shoots were obtained on MS medium supplemented with 1.00 mgl-1Kin in combination with 0.10 mgl-1 NAA. MS medium supplemented with 0.30 mgl-1 pyridoxine achieved the best shoot development compared with the other additive treatments and control. The highest rooting of shoots was with full strength MS medium supplemented with 1.00 mgl-1IBA and half strength MS medium with 1.50 mgl-1 NAA. Hardening the rooted cuttings was done in a greenhouse in pots containing a mixture of peatmoss, vermiculite and washed sand at the rate of 1:1:1 (v/v/v). Plantlets were successfully acclimated with 90% survival.