The aim of this study is to optimize in vitro multiplication of Coffea arabica cvs 'Benan',' Burai',' Odayni'
and 'Odayni- Bayat'. Seeds germinated on modified quarter MS medium with 1.0 g l−1 activated charcoal gave the
highest germination percentage of the four cultivars under study. BA at 8.0 mgl-1 was the most effective concentration
with an average of 14 shoots per explant, resulting in better and morphologically superior microshoots in 'Benan' cv.
However 'Burai' ,'Odayni' and 'Odayni- Bayat' cultivars, showed the highest number of shoots when they were grown
on MS medium supplemented with BA at 4.0, 2.0 and 6.0 mgl-1 respectively. Root formation was 100% in response to
the application of IAA, IBA and /or NAA to the culture media for the four cultivars. Half MS supplemented with NAA
at 2.0, 1.0 and 3.0 mgl-1 was found to be a suitable medium for root induction in excised micro-shoots, of 'Odayni',
'Burai' and 'Odayni-Bayat' cultivars, respectively. The plantlets were successfully acclimatized in the greenhouse;
consequently the survived plants reached 100%.