This exploration work target was to upgrade the micropropagation of potato cultivars through the utilization
of new light sources in the development rooms. The experiment consisted of two potato cultivars (Diamant and agria),
and five light sources (white, blue, green and red LEDs; and white fluorescent lights). The explants comprised of nodal
segments containing one bud, segregated from plantlets developed in vitro. The experimental design was completely
randomized arranged in a 2x5 factorial, with 4 replications. Each experimental unit consisted of a flask with five
explants. Three 28-day successive subcultures were done in MS medium and incubated under controlled conditions
(temperature = 25+2ºC; photoperiod = 16 hours; light intensity = 20 μmol m-2 s-1). Toward the finish of every
subculture, plantlet length, number of nodes per plantlet, internode length and plantlet fresh weight were assessed. After
the second subculture, differences were found among the two potato cultivars developed in vitro: "Agria" performed
better than "Diamant" in most plantlet growth parameters. Different light sources influenced the potato plantlet
development: red and green LEDs were the most and least prescribed for plantlet advancement respectively, in view of
the after effects of bud number per plantlet, plantlet length. Photosynthetic pigment contents were significantly different
under the different light treatments. Chlorophyll a, b and carotenoids were higher in plantlets grown under Red, and less
in plantlets under green LED light.