The effect of various treatments on the behavior of in vitro consecutive micropropagation stages of Capparis spinosa, L. was studied and a micropropagation protocol was developed. Three media were tested Murashige and Skoog (MS), Linsmaier and Skoog (LS) and Nitsch and Nitsch (NN) with shoot-tip and one node cutting for the establishment stage. Murashige and Skoog (MS) was found to be the best medium and one node cutting as starting materials for establishment from mother plants. Multiple shoots were obtained on MS medium supplemented with 0.50 mg/l 6-benzyladenine (BA) in the combination with 0.05 mg/l α-naphthaleneacetic acid (NAA). High frequency (85%) of rooting was obtained on 1/2MS semi solid medium supplemented with 1.5 mg/l Indole-3-butyric acid (IBA) alone or with 2g/l activated charcoal (AC). Regenerated plantlets were successfully transferred to pots containing mixture of peat-moss and vermiculite at equal volume with 90% survival.