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210948

The impact of addition of ascorbic acid to cryopreservation medium on dog epididymal spermatozoa

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Last updated: 03 Jan 2025

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Abstract

The objective of this study was to assess the effect of the addition of different concentrations of ascorbic acid to the extender on frozen-thawed epididymal dog spermatozoa. Epididymides from 17 castrated dogs were minced and incubated in a Tris buffer. The retrieved spermatozoa were diluted with Tris based-egg yolk-glycerol extender supplemented with different concentrations of ascorbic acid (0.45 mg/ml and 0.90 mg/ml) and the control (0.0 mg/ml). Diluted samples were equilibrated at 5°C for 2 h, packaged in 0.25 ml straws, and stored in liquid nitrogen (-196°C). After thawing (37°C for 30 s), sperm motility, viability, membrane integrity, acrosome integrity, DNA integrity, and lipid peroxidation by malondialdehyde (MDA) concentration were evaluated. The results were expressed as mean ± SE. Adding 0.90 mg/ml ascorbic acid to the cryopreservation medium significantly (P<0.05) improved motility, viability, membrane and acrosome integrity compared to the control. MDA concentration was significantly (P<0.05) reduced at 0.90 mg/ml related to the control. Percent of DNA damage was significantly (P<0.05) reduced in 0.45 mg/ml and 0.90 mg/ml ascorbic acid compared to the control. In conclusion, addition of ascorbic acid (0.90 mg/ml) to TCF extender resulted in a significant increase in the percentage of motility, viability, membrane intact, and acrosome- intact canine epididymal sperm, as well as the maintenance of DNA integrity and the reduction of lipid peroxidation at the membrane level.

DOI

10.21608/vmjg.2021.210948

Keywords

Dog, Epididymal sperm, cryopreservation, ascorbic acid, Oxidative Stress

Authors

First Name

Eman

Last Name

Fayez

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt

Email

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Orcid

-

First Name

Ali

Last Name

Salama

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt

Email

ash_andro@yahoo.com

City

-

Orcid

-

First Name

Zaher

Last Name

Rawash

MiddleName

M

Affiliation

Animal Reproduction Research Institute, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

El Sayed

MiddleName

A. I.

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt

Email

-

City

-

Orcid

-

Volume

67

Article Issue

1

Related Issue

23241

Issue Date

2021-12-01

Receive Date

2021-12-15

Publish Date

2021-12-01

Page Start

161

Page End

173

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_210948.html

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https://vmjg.journals.ekb.eg/service?article_code=210948

Order

14

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Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

The impact of addition of ascorbic acid to cryopreservation medium on dog epididymal spermatozoa

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Article

Created At

22 Jan 2023