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-Abstract
The present study will involve conducting surveillance for a zoonotic bacterial agent, Listeria monocytogenes in domestic animals and human in Egypt. The aim was to help in recording much more data concerning such disease and determining the most likely sources of Listeriosis infecting humans and the distribution of the various serotypes in the region. This information is important in order to optimize and determine appropriate intervention strategies and management of Listeriosis cases in animals especially ruminants and rabbits livestock. The results revealed that the occurrence of L. monocytogenes in the examined animal samples was 1.5 % (9/620) with high incidence in cows 1.3% (4/300) and in human acute blood samples was 0.8% (2/250). Most of the recovered isolates (72.7 %) showed high incidence of L. monocytogenes serotype 4 (8/11). Antibiogram was done for the recovered isolates and showed high resistance patterns for phenicols, polypeptides, cephalosporines and lincosamides. Genotypic analysis for the recovered isolates was carried out by using PCR with specific nucleotides sequence for prfA genes followed by sequencing and phylogenetic analysis. This analysis revealed that cows act as natural niches for that emerging pathogen.
DOI
10.21608/vmjg.2021.198764
Keywords
L. monocytogenes, surveillance, prfA gene, Sequencing, Phylogenetic analysis
Authors
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Department of Microbiology, Faculty of Veterinary Medicine, Cairo University
Email
ahmed.samir@cu.edu.eg
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Department of Microbiology, Faculty of Veterinary Medicine, Cairo University
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Animal Reproduction Research Institute, Agriculture research Center, Egypt.
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Department of Microbiology, Faculty of Veterinary Medicine, Cairo University
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Microbiology and Immunology Department, Veterinary Division, National Research Center (NRC), Egypt.
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Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, Egypt.
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-Link
https://vmjg.journals.ekb.eg/article_198764.html
Detail API
https://vmjg.journals.ekb.eg/service?article_code=198764
Publication Title
Veterinary Medical Journal (Giza)
Publication Link
https://vmjg.journals.ekb.eg/
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