A total of 410 milk samples were collected from different ruminant species as the following ; cows (n = 110),  buffaloes  (n = 87) ) , does (n=108) )  and ewes (n=105)  from different  Egyptian  farms .The results revealed that prominence of L.monocytogenes in the examined milk samples are 1.72 %  at  apparently healthy cow ,  2.77 % in  subclinical mastitic cow and in apparently healthy goat is 1.38 % and this has a public health importance. The recovered isolates identified biochemically as  L.monocytogenes  confirmed phenotypically by positive hemolytic reaction and motility , followed by molecular confirmation using specific primer sequences of 16s rRNA at 1200 bp followed with sequencing and accession on genbank . Antibiogram of the isolated L. monocytogenes are demonstrated resistance to tetracyclines, lincomycin, streptomycin, cefotaxime, Trimethoprime sulfamethoxazole, florphenicols, and clindamycin, while adherence assay for test glass tube polystyrene microtiter plates conclude that L. monocytogenes was able to form a strong biofilm.