Beta
ManageSearchChatDiscover
158007

Comparative Evaluation of Heat Shock and Electroporation Transformation of Canine Homlogue of HER2 Gene Cloned in a PUC57 Plasmid Construct

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Human epidermal growth factor receptor 2 (HER2) is an important prognostic marker in the case of breast cancer and other tumors. Canines remain one of the most important models for comparative oncology with humans due to the great similarity in the spontaneous presentation and development of cancer, and in the high homology in the amino acid sequence within the HER2 gene-encoded polypeptide. In this study, the canine homolog of the HER2 DNA sequence constructed in PUC57 plasmid was transformed in DH5α E.coli by heat-shock and electroporation. The plasmid was extracted from DH5α by Miniprep protocol and the HER2 gene 3738 bp was successfully amplified from PUC57 plasmid by polymerase chain reaction (PCR). Both heat shock and electroporation were efficient and resulted in the suspected amplicon. However, electroporation gave a higher transformation rate. HER2 DNA sequence will be used for the production of the recombinant HER2 protein in the near future.

DOI

10.21608/vmjg.2021.158007

Keywords

Recombinant HER2, breast cancer, electroporation, Heat shock, transformation

Authors

First Name

Karim

Last Name

Shokry

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Cairo University.

Email

-

City

-

Orcid

-

First Name

Rehab

Last Name

El Helw

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Cairo University.

Email

-

City

-

Orcid

-

First Name

Magdy

Last Name

Ghoneim

MiddleName

-

Affiliation

Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University.

Email

-

City

-

Orcid

-

First Name

Salah

Last Name

Selim

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, Cairo University.

Email

-

City

-

Orcid

-

Volume

67

Article Issue

1

Related Issue

23241

Issue Date

2021-12-01

Receive Date

2021-02-20

Publish Date

2021-12-01

Page Start

1

Page End

8

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_158007.html

Detail API

https://vmjg.journals.ekb.eg/service?article_code=158007

Order

1

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Comparative Evaluation of Heat Shock and Electroporation Transformation of Canine Homlogue of HER2 Gene Cloned in a PUC57 Plasmid Construct

Details

Type

Article

Created At

22 Jan 2023