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155932

Minor groove binder probe real-time RT-PCR for detection of foot-and mouth disease virus in Egypt

Article

Last updated: 03 Jan 2025

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Abstract

Shorter, more specific minor groove binders (MGBs) probes are dsDNA-binding agents attached to the 3' end of TaqMan probes that could be designed strictly to invariant region. Application and assessing of a new trend for viral detection in Egypt depending on MGB probe real-time RT-PCR (rRT-PCR) was applied on local FMDV serotypes O, A, and SAT2. Moreover, FMDV O were detected using two serotype specific primer sets by SYBR Green real-time RT-PCR assaying rapid formats. The limit of detection of diluted RNAs using MGB probe rRT-PCR assay reached to ≤ 6 fg/ul. Besides, high specificity of the former assay was clear. In contrary, the employing of FMDV O specific primer pairs in SYBR Green real-time RT-PCR showed a relative less sensitivity and specificity, particularly one of them displayed a very poor performance. Lastly, the local financial cost of MGB probe is considered the obvious hinder.

DOI

10.21608/vmjg.2018.155932

Keywords

Foot-and-Mouth disease virus, rRT-PCR, MGB probe

Authors

First Name

Hany

Last Name

Abu-Elnaga

MiddleName

-

Affiliation

Department of Foot and Mouth Disease, Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egyp PO Box 131. Postal No.11381.

Email

h.abu-elnaga@hotmail.com

City

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Orcid

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Volume

64

Article Issue

1

Related Issue

22953

Issue Date

2018-12-01

Receive Date

2018-01-01

Publish Date

2018-12-01

Page Start

1

Page End

13

Print ISSN

1110-1423

Online ISSN

2537-1045

Link

https://vmjg.journals.ekb.eg/article_155932.html

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https://vmjg.journals.ekb.eg/service?article_code=155932

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1

Type

Original Article

Type Code

544

Publication Type

Journal

Publication Title

Veterinary Medical Journal (Giza)

Publication Link

https://vmjg.journals.ekb.eg/

MainTitle

Minor groove binder probe real-time RT-PCR for detection of foot-and mouth disease virus in Egypt

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Type

Article

Created At

22 Jan 2023