The current study is an endeavor for profound understanding some hormonal and biochemical constituents during induction of ovulation in anestrous mares by using domperidone (Dopamine antagonist). Fifteen anestrous pure Arabian mares divided randomly into 2 groups, group (1) five mares and serve as a control, while Group (2) ten mares treated with 1.1 mg/kg domperidone for 25 days. Ultrasonographic scan was performed twice per week for detection of ovarian activity and follicular growth. Blood samples were collected one month before the treatment for 3 times with 10 days intervals for detection of progesterone, and twice per week after treatment till 10 days after the end of treatment for determination of progesterone, estrogen and prolactin hormones in addition to total protein, total cholesterol and triglycerides, before and after response for treatment. Eight mares (80%) showed ovulation and CL formation from the domperedone treated group while 2 mares (20%) showed no large follicles formation till the end of the trial period. The obtained results showed a significant increase in progesterone, estradiol and prolactin concentration in treated group compared to control one at 3rd and 4th week from treatment, while cholesterol level showed significant increase in treated group compared to control at 3rd week of treatment. And non significance change was recorded in protein and triglycerides levels. We can conclude that using Dopamine antagonist (Domperidone) is effective alternative for induction of ovulation in anestrous Arabian mares under Egyptian circumstances.