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248239

Development and Optimization of a Green Stability Indicating HPLC Method for the Determination Metformin HCL and Glibenclamide in their Dosage Form

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Analytical chemistry

Abstract

Green analytical chemistry approach was applied in attempt to develop, optimize and validate a stability indicating RP-HPLC method for the determination of metformin HCL and Glibenclamide in their combined dosage form. The chromatographic separation was performed on C18 column (250×4.6 mm, 5μm) at a λmax of 250 nm with a constant flow rate of 1.00 mL/minute. The isocratic mobile phase consisted of 70.00% organic (ethanol) to 30.00% aqueous 10.00 mM potassium phosphate buffer, pH 3.0 containing 50.00 mM octanesulphonic acid. The two drugs were stressed in acidic, basic, oxidative, and photolytic conditions. The developed method was validated according to ICH guidelines successfully. The detector response was linear in the concentration of 0.50-100.00 μg mL-1 for both drugs. Metformin HCL had detection limit and quantification limit of 0.15 g mL-1 and 0.50 g mL-1, while glibenclamide had detection limit and quantification limit of 0.16 g mL-1 and 0.49 g mL-1, respectively.

DOI

10.21608/rpbs.2022.141915.1148

Keywords

Green, stability, HPLC, Metformin HCL and Glibenclamide

Authors

First Name

Basma

Last Name

Selim

MiddleName

M.

Affiliation

Analytical chemistry, sinai university

Email

bsmamohamed41@gmail.com

City

-

Orcid

-

Volume

6

Article Issue

1

Related Issue

33246

Issue Date

2022-04-01

Receive Date

2022-05-30

Publish Date

2022-04-01

Page Start

84

Page End

100

Print ISSN

2536-9857

Online ISSN

2535-2091

Link

https://rpbs.journals.ekb.eg/article_248239.html

Detail API

https://rpbs.journals.ekb.eg/service?article_code=248239

Order

7

Type

Original Article

Type Code

532

Publication Type

Journal

Publication Title

Records of Pharmaceutical and Biomedical Sciences

Publication Link

https://rpbs.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023