A multidrug-resistant Staphylococcal species including Staphylococcus aureus and Staphylococcus epidermidis that colonizing patients with acne Vulgaris were investigated for the presence of resistance determinants. Acne vulgaris patients are usually subjected to topical antibiotic treatment including erythromycin and fusidic acid as the first line of treatments, which have been associated with resistance development. In this study, we attempt to investigate the dissemination of resistance determinants exclusively related to fusidic acid antibiotics mainly the horizontally transmitted fusB and fusC genes using the polymerase chain reaction technique. Our results suggested amplified resistance to fusidic acid with a large abundance magnitude of fusC gene among methicillin-resistant Staphylococcus aureus isolates, while an increased abundance of fusB gene among Staphylococcus epidermidis isolates compared to other resistance determinants. Conclusion: patients with acne vulgaris who were subjected to previous fusidic acid treatments should consider treatment with alternative antibiotics other than fusidic acid to achieve maximum treatment benefits considering clindamycin and aminoglycoside.