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273742

Protective effect of curcumin on diclofenac sodium-induced hepatotoxicity in male albino rats: Evidence of its antioxidant and anti-inflammatory properties

Article

Last updated: 22 Jan 2023

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Abstract

This research aims to explore if curcumin (CUR) can protect male albino rats from diclofenac (DIC) sodium-induced hepatotoxicity. The adult male Albino rats were divided into four groups (n=8) in this study. Control group: corn oil (i.p.) was given every day for seven days. CUR-treated group: was given CUR (200 mg/kg, i.p) every day for seven days. DIC-treated group: was given corn oil (i.p.) every day for seven days, followed by DIC single dose (150 mg/kg, i.p) 2 hours after last corn oil injection. DIC+CUR-treated group: was given CUR (200 mg/kg i.p.) every day for seven days followed by DIC single dose (150 mg/kg, i.p) two hours following the last CUR injection. Animals were sacrificed twenty-four hours following the last treatment. Blood serum was collected and tested for liver function markers. The liver was removed and used for biochemical and light microscopic examinations. The data showed that serum AST, ALT levels, as well as hepatic MDA, NO, and TNFα levels were significantly higher in the DIC-treated group than in the control group. While, serum total protein and albumin levels, as well as hepatic SOD levels, were significantly reduced. In contrast to the DIC-treated group, pretreatment with CUR resulted in a significant decrease in AST, ALT, MDA, NO, and TNFα as well as a significant increase in total protein, albumin, and SOD levels. CUR pretreatment also ameliorated the histological alterations. In conclusion, CUR treatment provided excellent protection against DIC-induced liver toxicity and oxidative damage in rats.

DOI

10.21608/rpbs.2022.177461.1189

Keywords

Diclofenac, Curcumin, Drug-induced hepatotoxicity, Oxidative Stress, TNF α

Authors

First Name

Eman

Last Name

Ali

MiddleName

Mohammed

Affiliation

Department of pharmacology, Faculty of Medicine, Sohag University

Email

emanmohamed@med.sohag.edu.eg

City

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Orcid

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Volume

6

Article Issue

3

Related Issue

33244

Issue Date

2022-04-01

Receive Date

2022-11-28

Publish Date

2022-04-01

Page Start

195

Page End

206

Print ISSN

2536-9857

Online ISSN

2535-2091

Link

https://rpbs.journals.ekb.eg/article_273742.html

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https://rpbs.journals.ekb.eg/service?article_code=273742

Order

273,742

Type

Original Article

Type Code

532

Publication Type

Journal

Publication Title

Records of Pharmaceutical and Biomedical Sciences

Publication Link

https://rpbs.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023