Truthfully, natural products have been the principal productive source of advancing new drugs. The broad aim of this work was to conduct a phytochemical and biological study of the Red Sea soft coral Sarcophyton glaucum, Family: Alcyoniidae collected from Red Sea at the Egyptian coasts. The phytochemical investigation from the soft coral S. glaucum led to the isolation of ten compounds including: palmitic acid (1), stearic acid (2), (24S)-24-methyl cholesterol (3), batyl alcohol (4), heptadecanoic acid pentadecyl ester (5), sarcophine (6), (+)-7α, 8β-dihydroxydeepoxysarcophine (7), uracil (8), thymine (9) and a ceramide (10). As cancer is one of the most hazardous factors threatening the human life, in this study the potential in-vitro cytotoxicity of the soft coral S. glaucum extract and three marine isolates were measured against HepG2 and MCF7 using Sulphorhodamine-B (SRB) assay and all of the tested samples showed a good cytotoxic activities against both hepatic and breast cancer. The extract of S. glaucum was tested also against the protozoan parasite Leishmania donovani, using pentamidine and amphotericin B as controls and showed antileishmanial activity.