Ciprofloxacin belongs to the fluoroquinolone family, which exhibits biological activity against both gram-positive and gram-negative bacteria. The previously mentioned drug is of utmost importance due to the high incidence of resistance caused by other antibiotics. Thus, numerous research studies has been conducted to determine Ciprofloxacin as well as its metabolites owing to their contribution in the drug's biological activity. Liquid chromatography, gas chromatography, capillary electrophoresis and other separation techniques were used to develop, adapt and implement analytical techniques for the drug analysis. Other studies has made use of the hyphenation between liquid chromatography and mass spectrometry for Ciprofloxacin assay for quality control, biological fluids, animal tissues and numerous sample types. The recent advances in the ionization methods has led to the use of direct mass spectrometry techniques to analyze various sample types. This mini review aims to focus on the various analytical techniques and recent technologies implemented for Ciprofloxacin analysis.