The field of trace analysis of analytes in different samples has gained an increasing interest. This field of analysis gained many benefits by entering the era of nanomaterials and nanotechnologies. One aspect of nanotechnologies influence on the field of trace analysis is the development of magnetic dispersive micro-solid phase extraction (Magnetic D-µ-SPE) technique. Magnetic D-µ-SPE technique involves dispersion of an adsorbent phase with special magnetic properties as finely divided form; usually as nanoparticles, in sample solutions to adsorb target analytes. This is followed by adsorbent retrieval by external magnetic field. This technique provided several advantages including minimal use of solvents and adsorbent materials as well as procedures simplicity. An important key element in the preparation of Magnetic D-µ-SPE adsorbents is tailoring of its magnetic properties. Superparamagnetic nanoparticles had unique properties which fulfilled requirements of Magnetic D-µ-SPE procedures. This review discusses superparamagnetic behavior and its application in preparation of adsorbents for Magnetic D-µ-SPE technique.