The volatiles of both fresh aerial parts and flowers of Carthamus lanatus L. plant growing in Egypt were isolated by hydro-distillation method. The obtained volatiles were subsequently subjected to qualitative analysis by Gas chromatography accompanied by mass specctrometic analysis (GC-MS) , which in turn revealed the presence of forty four compounds. The interpretation of the isolated compounds was carried out depending on comparison of their mass spectra with those saved in instrument library, alongside the data reported in literature. On the other hand, a comparative investigation between the Egyptian and Bulgarian species was carried out, which in turn revealed great differences. Antimicrobial screening (using agar diffusion method) of the screened volatiles of Egyptian plant was also carried out, that labored into promising results of potent activity exhibited against different bacteria and fungi compared with gentamycin and ketoconazole antimicrobial standards. it is worth mentioned that our study is the first study of the Egyptian Carthamus lanatus Plant.