A number of methods of sampling the vagina for the presence of candida have been developed. Such techniques play an important role in the diagnosis and management of vaginal candidiasis. In the past, identification of candida isolated from the vagina has usually been limited to the genus Candida or to the species C. albicans. However, with the recognition that Candida species differ in the production of putative virulence factors and sensitivity to antifungal agents, greater emphasis has been placed on identification of isolates to species level. As a result, a range of commercially available systems for yeast identification can now be used in conjunction with traditional identification procedures. In this work, Candida detected by many phenotypic identification methods such as sabaroud dextrose agar medium, microscopical identification, sugar fermentation test and germ tube test. Polymerase chain reaction (PCR) also used as a fast and sensitive genotypic technic to identify Candida sp.