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208190

Soil actinomycetes molecular characterization for secondary metabolites production

Article

Last updated: 03 Jan 2025

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Subjects

-

Tags

Microbiology

Abstract

Antibiotic resistance issue is really unavoidable, increased due to decrease in production of novel secondary metabolites production. Actinomycetes have the potential to produce biological active compounds used as an antibacterial, antiviral, antifungal, antiprotozoal, antihelminthic, anticholesterol, anticancer, immunosuppressant, pesticides and herbicides. Current research study was design to assess actinomycetes for production secondary metabolites. For this purpose different soil samples were collected and with help of dilution method, actinomycetes were grown on enrichment. Actinomycetes were morphologically analysed under microscope and molecularly through PCR and 16S-rRNA gene-amplification. After confirmation, actinomycetes were grown on LB and GMC media have potential to produce bioactive secondary metabolites. Current research study results that actinomycetes have potential to produce bioactive secondary metabolites. LB-media is the best suitable-medium for growth of actinomycetes and bioactive secondary-metabolites production. It is suggested that actinomycetes strains from various environment should be assessed for novel bioactive secondary metabolites.

DOI

10.21608/jabps.2021.98256.1141

Keywords

Actinomycetes, antibiotics, 16s rRNA gene, PCR, Streptomyces

Authors

First Name

Shakir

Last Name

Ullah

MiddleName

-

Affiliation

Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Pakistan.

Email

shakir.ullah1210@gmail.com

City

-

Orcid

-

First Name

Zubaida

Last Name

Muhammad

MiddleName

Sher

Affiliation

Department Zoology, Sardar Bahadur Khan Women University Quetta Baluchistan

Email

rozinarashid10@gmail.com

City

-

Orcid

-

First Name

Saqlain

Last Name

Jehan

MiddleName

-

Affiliation

Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Pakistan.

Email

saqlainjehann@gmail.com

City

-

Orcid

-

First Name

Samavia

Last Name

Zia

MiddleName

-

Affiliation

Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Pakistan.

Email

samaviazia746@gmail.com

City

-

Orcid

-

First Name

Zakir

Last Name

Hussain

MiddleName

-

Affiliation

Department of Chemistry, Government College University Faisalabad Pakistan

Email

biologyacademy0@gmail.com

City

-

Orcid

-

First Name

Syed

Last Name

Hussain

MiddleName

Amjad

Affiliation

Department of Chemistry, The university of Punjab Lahore Pakistan

Email

sareerudin121@gmail.com

City

-

Orcid

-

First Name

Ramiz

Last Name

Shirazi

MiddleName

Raza

Affiliation

Department of Chemistry, Government College University Faisalabad Pakistan

Email

ramizraja1211@gmail.com

City

-

Orcid

-

First Name

Asad

Last Name

Ullah

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, Pakistan.

Email

asadullah461236@gmail.com

City

-

Orcid

-

First Name

Abdur

Last Name

Raziq

MiddleName

-

Affiliation

Department of Bioinformatics & Biotechnology, Faculty of Life Science, Government College University Faisalabad, Pakistan.

Email

bsadia962@gmail.com

City

-

Orcid

0000-0003-2779-3269

First Name

Anbareen

Last Name

Gul

MiddleName

-

Affiliation

Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

Email

h_hayat2015@yahoo.com

City

-

Orcid

-

First Name

Hayat

Last Name

Ullah

MiddleName

-

Affiliation

Government College University Faisalabad

Email

hayatullah22@gcuf.edu.pk

City

Punjab

Orcid

0000-0002-9463-8528

Volume

5

Article Issue

1

Related Issue

29743

Issue Date

2022-01-01

Receive Date

2021-09-27

Publish Date

2022-01-01

Page Start

40

Page End

44

Print ISSN

2535-1869

Online ISSN

2535-2040

Article File

JABPS_Volume 5_Issue 1_Pages 40-44.pdf

PDF . 423.9kB

Link

https://jabps.journals.ekb.eg/article_208190.html

Detail API

https://jabps.journals.ekb.eg/service?article_code=208190

Order

4

Type

Original Article

Type Code

522

Publication Type

Journal

Publication Title

Journal of advanced Biomedical and Pharmaceutical Sciences

Publication Link

https://jabps.journals.ekb.eg/

MainTitle

Soil actinomycetes molecular characterization for secondary metabolites production

Details

Type

Article

Created At

22 Jan 2023