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208187

Role of apoptosis and oxidative stress in high glucose-induced endothelial dysfunction in isolated aortic rings

Article

Last updated: 23 Dec 2024

Subjects

-

Tags

Pharmacology and Toxicology

Abstract

Diabetes and its associated hyperglycemia represent a major health problem. One of the common complications of diabetes is endothelial dysfunction which leads to a number of cardiovascular diseases. Studying of the effect of hyperglycemia and possible treatment strategies necessities the induction of diabetes in vivo requires a long time for endothelial dysfunction to develop. Recently, in vitro models of hyperglycemia have been introduced in which vascular tissues are incubated in media containing high concentration of glucose which ultimately requires much less time for endothelial dysfunction development. In this study, we examined the effect of incubation of isolated aortic rings in a high glucose medium on their relaxation and contractile responses as well as the histopathological changes of aortic tissues. We also examined the involvement of oxidative stress and apoptosis in hyperglycemia induced endothelial dysfunction. The results of our study showed that incubation of aortic rings lead to a significant reduction of relaxation response of precontracted aortic rings along with an enhanced contractile response to epinephrine. This was associated with obvious histopathological alterations of aortic tissues. Aortic rings incubated in high glucose also exhibited an increased oxidative stress and a reduction of the anti-apoptotic marker, Bcl2. In conclusion, in vitro high glucose can be used as a model for studying endothelial dysfunction and its possible mechanisms involving oxidative stress and apoptotic pathway.

DOI

10.21608/jabps.2021.95071.1139

Keywords

High Glucose, endothelial dysfunction, Oxidative Stress, apoptosis, Vascular complications

Authors

First Name

Eman

Last Name

Awad

MiddleName

-

Affiliation

Department of Pharmacology and Toxicology, Minia University

Email

eman_moftah90@yahoo.com

City

-

Orcid

-

First Name

Al-Shaimaa

Last Name

Ahmed

MiddleName

F.

Affiliation

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Egypt

Email

shaimaa.faissal@minia.edu.eg

City

Minia

Orcid

0000-0001-9875-6399

First Name

Mahmoud

Last Name

El-Daly

MiddleName

-

Affiliation

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, El-Minia 61519, Egypt

Email

eldaly_m@mu.edu.eg

City

-

Orcid

0000-0001-5971-1104

First Name

Nashwa

Last Name

El-Tahawy

MiddleName

-

Affiliation

Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Egypt

Email

nashwa.abdo@minia.edu.eg

City

-

Orcid

0000-0003-2660-9909

First Name

Alshaimaa

Last Name

Kasem

MiddleName

-

Affiliation

Department of Pathology, Faculty of Medicine, Minia University

Email

dralshaimaawagdy@gmail.com

City

-

Orcid

-

First Name

Ashraf

Last Name

Taye

MiddleName

-

Affiliation

Department of Pharmacology and Toxicology, Faculty of Pharmacy, South Valley University

Email

ashraf_taye70@yahoo.com

City

-

Orcid

-

Volume

5

Article Issue

1

Related Issue

29743

Issue Date

2022-01-01

Receive Date

2021-09-12

Publish Date

2022-01-01

Page Start

23

Page End

28

Print ISSN

2535-1869

Online ISSN

2535-2040

Link

https://jabps.journals.ekb.eg/article_208187.html

Detail API

https://jabps.journals.ekb.eg/service?article_code=208187

Order

2

Type

Original Article

Type Code

522

Publication Type

Journal

Publication Title

Journal of advanced Biomedical and Pharmaceutical Sciences

Publication Link

https://jabps.journals.ekb.eg/

MainTitle

Role of apoptosis and oxidative stress in high glucose-induced endothelial dysfunction in isolated aortic rings

Details

Type

Article

Created At

22 Jan 2023