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The Utility of Acetylbutyrolactone for Spectrofluorimetric Determination of Two Gamma-Aminobutyric Acid Analogues

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Last updated: 03 Jan 2025

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Tags

Analytical and Medicinal Chemistry

Abstract

Acetylbutyrolactone was applied as a fluorogenic reagent for developing a simple, selective and economic method for determination of Two Gamma-Aminobutyric Acid (GABA) analogues containing primary aliphatic amine group (gabapentin and pregabalin). The reaction depends on the formation of Schiff bases which have strong fluorescence activities at 440 nm after excitation at 380 nm. The different reaction parameters that affect the stability of the formed Schiff base have been deeply investigated and optimized. The suggested reaction mechanism has been explained. The fluorescence-concentration plots were rectilinear over the range of 20-100 µg ml-1 for gabapentin and 10-100 µg ml-1 for pregabalin with excellent correlation coefficients (0.9998 for Gabapentin and 0.9999 for pregabalin). The lower quantification limits were 2.9 and 1.9 µg ml−1 for gabapentin and pregabalin, respectively. According to ICH guidelines, the method was validated and the results were in agreement with other reported methods in respect to accuracy and precision. Determination of gabapentin and pregabalin in the commercial capsules were achieved using the proposed method with excellent recoveries (100.22% for Gaptin® capsules and 100.28% for Lyrolin® capsules) and high degree of accuracy.

Keywords

Gabapentin, Pregabalin, Acetylbutyrolactone, Spectrofluorimetry

Authors

First Name

Sayed

Last Name

Derayea

MiddleName

M.

Affiliation

Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519-Minia, Egypt

Email

sayed_derayea@yahoo.com

City

-

Orcid

-

First Name

Tamer

Last Name

Attia

MiddleName

Z.

Affiliation

Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519-Minia, Egypt

Email

tamer_zekry_a@yahoo.com

City

-

Orcid

-

First Name

Mohammed

Last Name

El Nady

MiddleName

-

Affiliation

Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519-Minia, Egypt

Email

mohammedelnady73@yahoo.com

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

1479

Issue Date

2018-06-01

Receive Date

2018-04-10

Publish Date

2018-06-01

Page Start

6

Page End

12

Print ISSN

2535-1869

Online ISSN

2535-2040

Article File

JABPS_Volume 1_Issue 1_Pages 6-12.pdf

PDF . 936.0kB

Link

https://jabps.journals.ekb.eg/article_7668.html

Detail API

https://jabps.journals.ekb.eg/service?article_code=7668

Order

2

Type

Original Article

Type Code

522

Publication Type

Journal

Publication Title

Journal of advanced Biomedical and Pharmaceutical Sciences

Publication Link

https://jabps.journals.ekb.eg/

MainTitle

The Utility of Acetylbutyrolactone for Spectrofluorimetric Determination of Two Gamma-Aminobutyric Acid Analogues

Details

Type

Article

Created At

22 Jan 2023