The goal of the study is to examine the action of Curcumin-Chitosan (Cur-Chito) nanocomposite supplementation against Carbon tetrachloride toxicity (liver fibrosis model) induced testis impairment. Four groups of 60 mice were under investigation; normal control, Carbon tetrachloride, prevention, and treatment group. Mice were IP injected with Carbon tetrachloride (0.5 ml/kg body weight) 3 times /week x4 weeks to induce liver fibrosis injury. While mice were orally administrated Cur-Chito nanocomposite (100ml/k.g. body weight) 5 times/week x 4 weeks as a style of prevention and treatment. The physiological and biochemical analysis, testis and body weights, semen concentration, were all recorded. The treatment with Carbon tetrachloride showed extensive collagen fibers in and around the portal areas in the liver tissue, atrophy of the seminiferous tubules, disrupted basement membrane, decreased sperm count, decreased testis weight, and body weight in the male reproductive system. Improvement in the histological examination (liver and testis tissues), sperm count, and oxidative status in the Cur-Chito nanocomposite administrated groups in comparison to the Carbon tetrachloride administrated groups. In conclusion (Cur-Chito) nanocomposite can prevent and treat testicular complications of liver and testis tissues in Carbon tetrachloride treated mice.