Beta
219346

Effect of cryoprotectants on camel oocytes Vitrification

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Zoology

Abstract

The objective of this study was to show how two cryoprotectants affect the vitrification rate in immature camel oocytes when used in a separate or a mixed form. As a result, their ability to mature after being warmed was recorded. The cumulus oocytes complexes (COCs) were divided into four groups, control; EG (30 percent ethylene glycol) in vitrification solution (VS); DMSO (30 percent dimethyl sulfoxide); MIX (15 percent EG + 15 percent DMSO) in vitrification solution (VS). COCs were impeded in equilibration solution (ES) for 2 minutes before being transferred to a vitrification solution containing 30% cryoprotecting agent (EG, DMSO, or MIX), 20% FBS, and 0.5 M sucrose in the medium in each vitrification group. Oocytes were put into the open pulled straw (OPS) and subsequently frozen for one hour in liquid nitrogen (LN2). The oocytes were then thawed and warmed for 2.5 minutes at 37°C in four warming solutions (WS) containing varying amounts of sucrose. The findings revealed that the oocyte maturation rate was high. In comparison, DMSO treatment had a better recovery rate, viability, and more negligible damage effect than EG and MIX.

DOI

10.21608/absb.2022.111247.1161

Keywords

Vitrification, cryoprotectants, oocytes, Dromedary camel, IVM

Authors

First Name

Hagar

Last Name

Yassin

MiddleName

B.

Affiliation

Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls).

Email

hagarbadawy2018@yahoo.com

City

El-Fayoum

Orcid

-

First Name

Nehal

Last Name

Abu-Elnaga

MiddleName

A.

Affiliation

Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls).

Email

naboulnaga61@gmail.com

City

Cairo

Orcid

0000-0001-5599-4733

First Name

Bahaa

Last Name

Farrag

MiddleName

-

Affiliation

Animal and Poultry Production Division, Desert Research Center, 11753, Cairo, Egypt.

Email

drc_bahaa@yahoo.com

City

Cairo

Orcid

0000-0003-2535-3681

First Name

Khalid

Last Name

El-Bahrawy

MiddleName

A.

Affiliation

Animal and Poultry Production Division, Desert Research Center, 11753, Cairo, Egypt.

Email

bhrawi@yahoo.com

City

Alexandria

Orcid

0000-0001-7173-0340

Volume

33

Article Issue

Issue 1-C

Related Issue

36491

Issue Date

2022-06-01

Receive Date

2021-12-23

Publish Date

2022-06-01

Page Start

91

Page End

104

Print ISSN

1110-2535

Online ISSN

2636-3305

Link

https://absb.journals.ekb.eg/article_219346.html

Detail API

https://absb.journals.ekb.eg/service?article_code=219346

Order

8

Type

Original Article

Type Code

520

Publication Type

Journal

Publication Title

Al-Azhar Bulletin of Science

Publication Link

https://absb.journals.ekb.eg/

MainTitle

Effect of cryoprotectants on camel oocytes Vitrification

Details

Type

Article

Created At

22 Jan 2023