Sewage is the primary source of faecal contamination of groundwater with pathogenic bacteria. Protecting groundwater
from microbial contamination is a top public health priority, This study deals with evaluation of groundwater wells, ground
water well based-plants, in Alfath lying in Eastern area of Nile Basin, Assuit at summer 2014 with assessment of presence of
thermotolerant faecal coliform and thermotolerant faecal streptococci bacteria as a potent indicators for faecal contamination,
we used a multiple-tube fermentation or membrane filtration for enumerating themotolerant faecal coliform and thermotolerant
faecal streptococci using most-probable-number (MPN) index. This study consisted of a total of 75 water samples obtained
for routine testing at summer 2014, there are 5 water wells not suitable consuming as drinking water for human, 3 expulsion of
water well plants (Alfath-Alkadema, Bani-Talib and Arab-Moteer) need to sterilization or replacement and renovation. Physicochemical
parameters were determined for these water samples.