Electrophoretic profiles of native seed protein and esterase isozyme characters of four species of genus Mimusops have been revealed by PAGE. Seed coat scanning and numerical analysis of the results carried out. These species are M. laurifolia, M. elengi,M. zeyheri and M. coffra. The results of SEM on the surface seeds showed that, Seed ornamentation in Mimusops zeyheri differs than the rest species of the genus Mimusops by showing replicas of freeze on the their membrane surface. The results of seed protein and esterase isozyme characters indicated that delimitation of Mimusops zeyheri in a separated level, separation of two species of Mimusops coffra and Mimusops elengi in a separated group, in addition  distinguishing of Mimusops laurifolia than other two species of Mimusops elengi and Mimusops coffra.