An actinomycete culture could be isolated from a soil sample collected from Bani-Swife governorate, Egypt. The isolate AZ-B2 produced a wide spectrum antibiotic (anti-Gram-positive and Gram-negative bacteria). The isolate AZ-B2 matches with Streptomyces griseoviridis in the morphological, physiological and biochemical characters. Thus, it was given the suggested nameStreptomyces griseoviridis.The parameters controlling the biosynthetic process of antibacterial agent formation including different pH values, different temperatures, different incubation period, and different carbon and nitrogen sources were fully investigates.                 The separation of the active ingredient and its purification was performed using both thin layer chromatography (TLC) and column chromatography (CC) techniques. The physico-chemical characteristics of the purified antibiotic viz. color, melting point, solubility, elemental analysis, spectroscopic characteristics and chemical reactions have been investigated. This analysis indicates a suggested imperical formula ofC₁₈H₃₆N₄O₁₁. The minimum inhibition concentrations "MICs" of the purified antibiotic were also determined. The collected data emphasized the fact that the purified antibiotic compound was suggestive of being belonging to Aminocyclitol group (Kanamycin A antibiotic) produced by Streptomyces griseoviridis, AZ- B2