Some fungal species isolated from the soil of the radioactive repository site at HotLaboratoriesCenter were investigated for their tolerance, affinity and capacity towards different metal ions namely; Cerium Ce(III), Chromium Cr(VI), Cobalt Co(II), and Cadmium Cd(II). They were identified as Aspergillus tamarii, Aspergillus niger, Penicellium chrysogenum, Cunninghamella elegans and Rhizopus stolonifer. The obtained results showed that, the lowest concentration of metal ion could be tolerated by fungal species was 50ppm for Co(II) & Cd(II) , while the highest one was 2000ppm for Cr(VI) & Ce(III). Also, tolerance towards mixture of ions was varied among the fungi under study. The affinity of the fungal species of study for metal ions under investigation was found to have the following order; Ce(III)>Cd(II)>Co(II)>Cr(VI). Results showed that Aspergillus niger had high capacity for Ce(III) ions uptake, while Cunninghamella elegans had high capacity for Co(II) ionsuptake. The effects of chemical and physical pretreatments of fungal biomass on the uptake capacity were studied using Aspergillus niger & Ce(III). The uptake of Ce(III) by Aspergillus niger was studied using different concentrations of Ce(III). It was found that one gram dry weight of Aspergillus niger could accumulate 285.7 ± 16.9, 571.43 ± 23.9 and 1142.86 ± 33.8 mg of Ce(III) from 50, 100 and 200 ppm solutions, respectively.