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FRACTIONAL FACTORIAL DESIGN APPROACH FOR STUDYING IN VITRO TRANSDERMAL DELIVERY OF GRANISETRON USING ELECTROPORATION

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Last updated: 03 Jan 2025

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Chemistry

Abstract

Granisetron Hydrochloride is a 5-hydroxytryptamine 3 (5-HT3) receptor antagonist used for the prevention and treatment of nausea and vomiting associated with cancer chemotherapy. Based on the hydrophilicity of this drug (pka=9.4) it is unlikely that passive diffusion across the skin could deliver therapeutic amounts from a large sized patch. This study was conducted to evaluate the feasibility of in vitro transdermal delivery of granisetron using electroporation across full thickness hairless rat skin. A 24-1fractional factorial design was used to determine the most important variables affecting the transdermal delivery of granisetron using electroporation. The variables studied and respective levels investigated were drug concentration (10, 20 mg/ml), voltage (155, 310 volt), pulse duration (12, 24 ms) and number of pulses (100, 200 pulses). Permeation profiles were used for the representation of data, where Q: the cumulative amount permeated per cm2 (µg/cm2) was plotted against time (min). The selected dependent variable (response) was Q120 which is the cumulative amount permeated per cm2 after 2 hours. Design-expert version 7.0.0 was used for the statistical evaluation. The results of the analysis of variance (ANOVA) revealed that Q120 was significantly affected by the voltage, pulse duration and number of pulses. The drug concentration had a non significant effect on Q120. A reduced polynomial regression equation which expresses the influence of process parameters on the response was obtained to enable navigation of the experimental space.

DOI

10.21608/absb.2008.10089

Keywords

Granisetron, Transdermal, electroporation, Fractional factorial design

Authors

First Name

SAMIA

Last Name

NOUR

MiddleName

A.

Affiliation

Department of Pharmaceutics, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Email

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City

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Orcid

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First Name

MOHAMED

Last Name

ELEWA

MiddleName

T.

Affiliation

Department of Electrical Engineering, Faculty of Engineering, Benha University , Egypt.

Email

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City

-

Orcid

-

First Name

Randa

Last Name

Abd El-rehim

MiddleName

T.

Affiliation

Department of Pharmaceutics, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Email

randa_tag@yahoo.co.uk

City

-

Orcid

-

Volume

19

Article Issue

Issue 1-A

Related Issue

1639

Issue Date

2008-06-01

Receive Date

2008-02-01

Publish Date

2008-06-01

Page Start

245

Page End

267

Print ISSN

1110-2535

Online ISSN

2636-3305

Article File

ABSB_Volume 19_Issue Issue 1-A_Pages 245-267.pdf

PDF . 1.7MB

Link

https://absb.journals.ekb.eg/article_10089.html

Detail API

https://absb.journals.ekb.eg/service?article_code=10089

Order

17

Type

Original Article

Type Code

520

Publication Type

Journal

Publication Title

Al-Azhar Bulletin of Science

Publication Link

https://absb.journals.ekb.eg/

MainTitle

FRACTIONAL FACTORIAL DESIGN APPROACH FOR STUDYING IN VITRO TRANSDERMAL DELIVERY OF GRANISETRON USING ELECTROPORATION

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Type

Article

Created At

22 Jan 2023