Ascaris lumbricoides remains the most common intestinal nematode in the world. The cytokine are considered the arm of the adaptive immune system is mediated by T helper cells (Th1and Th2) and related cytokines, including interferon (IFN)-γ and tumor necrosis factor (TNF)-α, and has been reported to play a pivotal role in the control of many parasites . This study investigates the antihelmintic effect of natural product (Allicin garlic) extracts and also synthetic drugs (Piperazine Citrate ). Our target was to study the effect of natural and synthetic drugs on immune induction of infected rats by Ascaris lumbricoides . Ninety male rats Rattus Rattuswere used. The rats were divided into 7 groups. The 1st group was control clean, and the second group was infected control . The groups ( 3, 4, 5,and 6 ) were treated by Allicin garlic extracts i.m 8 times twice weekly at 0.1, 0.2 , 0.3 and 0.4 mg/kg respectively). The Seventh group received Piperazine Citrate drug at dose 10 mg/kg body weight 8 times twice weekly. All groups were scarified after 30 days and investigated with the immunoresponse (cytokine) IL-10 and TNF – alpha, paralleled with measurements the ALT, AST, ALP, GGT and Malondialdehyde as an immune factor . The results of the current study revealed that the levels of IL-10 and TNF- alpha were increased significantly in groups treated with natural product (Allicin garlic) . also our data reveled that the MDA level was decreased significantly in groups treated with natural product (Allicin garlic) . On the other hand liver function ALT , AST , ALP and GGT were reveled the significant increased in groups treated by natural product (Allicin garlic as well asPiperazine Citrate drug .