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7561

STUDIES ON THE INDUCTION OF LIPASE(S) BY THERMOALKALOPHILIC BACILLUS bREVIS B2

Article

Last updated: 22 Jan 2023

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Subjects

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Tags

Botany
Microbiology
Zoology

Abstract

Bacillus brevis-B2 was selected as the most patent lipase(s) producer among nine thermoalkalophilic bacterial isolates isolated from agro industrial wastes at 70oC and pH 10.5. Of the tested nutrient medium, tap water containing 1% fish wastes and 1% yeast extract is the best inducer for lipase synthesis by B. brevis B2. Maximum enzyme biosynthesis could be obtained after 24 hr of incubation. The optimal pH and temperature for enzyme formation are 11.5 and 70oC. Enzyme yield increased in presence of sucrose or lactose in the nutrient medium, also the presence of 100 ppm zinc sulphate and 500 ppm folic acid supported high induction of lipase by B. brevis growing under shaking condition and inocula size of 2ml/100ml culture medium. A very slight increase in enzyme formation occur under exposure of bacterial isolate to 1 KGy of γ-irradiation.

DOI

10.21608/absb.2009.7561

Keywords

Thermoalkalophilic bacteria, Lipase, physiology

Authors

First Name

EGLAL

Last Name

GHONEIMY

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.

Email

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City

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Orcid

-

First Name

REDA

Last Name

BAYOUMI

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

SANAA

Last Name

AHMED

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

NAHID

Last Name

YOUNIS

MiddleName

A.

Affiliation

Nuclear Research Center, Atomic Energy Authority, Egypt.

Email

-

City

-

Orcid

-

First Name

OLA

Last Name

EL-HAGER

MiddleName

E.

Affiliation

Nuclear Research Center, Atomic Energy Authority, Egypt.

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-

City

-

Orcid

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Volume

20

Article Issue

Issue 2-C

Related Issue

1452

Issue Date

2009-12-01

Receive Date

2009-08-09

Publish Date

2009-12-01

Page Start

1

Page End

20

Print ISSN

1110-2535

Online ISSN

2636-3305

Article File

ABSB_Volume 20_Issue Issue 2-C_Pages 1-20.pdf

PDF . 221.2kB

Link

https://absb.journals.ekb.eg/article_7561.html

Detail API

https://absb.journals.ekb.eg/service?article_code=7561

Order

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Type

Original Article

Type Code

520

Publication Type

Journal

Publication Title

Al-Azhar Bulletin of Science

Publication Link

https://absb.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023