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VALIDATED STABILITY INDICATED RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF METHYL SALICYLATE, CAMPHOR AND MENTHOL IN CREAM PREPARATIONS

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Last updated: 03 Jan 2025

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Chemistry

Abstract

A method for the determination of three active products in pharmaceutical preparation Rheumatizen topical cream was described. The method was based on isocratic elution of Methyl Salicylate, Camphor and Menthol on reversed phase column C8 Thermo hyperseal 250mm x 4.6mm 5µm - using a mobile phase consisting of mixture of 45% Acetonitrile: 55% (Water+0.2% Triethyl amine adjusted to pH 5 with acetic acid)at a flow rate of 1 ml/min. Quantization was achieved with Refractive index (RI) detector. Linearity of Methyl Salicylate, Camphor and Menthol was found to be from (2.4 mg/ml to 9.6 mg/ml), (0.32 mg/ml to 1.28 mg/ml) and (0.8 mg/ml to 3.2 mg/ml), with variation coefficient 0.9998, 0.997 and 0.998 respectively. The stress testing was carried out by using the solutions that had been treated with [2M NaOH, 2M HCl and 30%H2O2] and all of these solutions were leaved for 22 hours at room temperature in tightly closed containers without further heating to avoid the loss via evaporation of the analyets due to their volatility character.

DOI

10.21608/absb.2012.7204

Keywords

Methyl salicylate, Camphor,Menthol, analgesic, anti inflammatory, Reversed phase, refractive index, HPLC, drug analysis

Authors

First Name

I.

Last Name

ABD ELLAH

MiddleName

M.

Affiliation

Department of chemistry, faculty of science, Al-Azher University, Naser city, Cairo, Egypt

Email

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City

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Orcid

-

First Name

M.

Last Name

ELNAWAWY

MiddleName

-

Affiliation

Department of chemistry, faculty of science, Al-Azher University, Naser city, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

A.

Last Name

ELEWA

MiddleName

M.

Affiliation

Methodology department, Medizen pharmaceutical industries, Borg-Alarab, Alexandria, Egypt

Email

-

City

-

Orcid

-

Volume

23

Article Issue

Issue 2-A

Related Issue

1369

Issue Date

2012-12-01

Receive Date

2012-05-14

Publish Date

2012-12-01

Page Start

45

Page End

60

Print ISSN

1110-2535

Online ISSN

2636-3305

Article File

ABSB_Volume 23_Issue Issue 2-A_Pages 45-60.pdf

PDF . 629.8kB

Link

https://absb.journals.ekb.eg/article_7204.html

Detail API

https://absb.journals.ekb.eg/service?article_code=7204

Order

3

Type

Original Article

Type Code

520

Publication Type

Journal

Publication Title

Al-Azhar Bulletin of Science

Publication Link

https://absb.journals.ekb.eg/

MainTitle

VALIDATED STABILITY INDICATED RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF METHYL SALICYLATE, CAMPHOR AND MENTHOL IN CREAM PREPARATIONS

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Type

Article

Created At

22 Jan 2023