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VARIATION OF PROTEIN PROFILE AMONG CONSECUTIVE STINGS OF THE SCORPION PARABUTHUS LEIOSOMA (FAMILY: BUTHIDAE) FROM EGYPT, SUPPORTS THE VENOM-METERING HYPOTHESIS IN SCORPIONS.

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Last updated: 03 Jan 2025

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Botany
Microbiology
Zoology

Abstract

Scorpions use their venom to immobilize prey items and defend themselves against predators. Scorpion venom is a complex mixture composed of a wide array of substances such as salts, small molecules, peptides, and proteins. During analysis of the prey capture behavior of the scorpion Parabuthus leiosoma, we noticed that the scorpion stings the prey several successive stings in different specific regions. The stings started with ventral surface of the abdomen followed by thorax, shoulders and finally the head. This finding raised the possibility that the scorpion might use the venom with different composition in different contexts. To check this possibility, venom was collected as sequential drops (stings) by electrical stimulation (20 Volt) of the telson of the Parabuthus leiosoma. Protein content was determined by spectrophotometer using total protein KIT. The results showed marked variation of the total protein among the consecutive stings of each of the investigated scorpions. To go further, the protein profiles for all the collected stings were analyzed separately using 10% SDS-polyacrylamide electrophoresis (SDS-PAGE). Similarly, differences were observed among the stings, not only in the number of the protein bands, but also in the intensity of the bands. Thus, these results indicated that different venom components are unequally represented among consecutive stings supporting the venom-metering hypothesis.

DOI

10.21608/absb.2012.7209

Keywords

Scorpion, Venom, Stinging, Consecutive stings, Electrophoresis, Parabuthus leiosoma

Authors

First Name

MOUSTAFA

Last Name

SARHAN

MiddleName

M.

Affiliation

Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.

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City

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Orcid

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First Name

MAGED

Last Name

FOUDA

MiddleName

M.

Affiliation

Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.

Email

-

City

-

Orcid

-

First Name

ALAA

Last Name

ELBITAR

MiddleName

M.

Affiliation

Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.

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-

City

-

Orcid

-

First Name

HAMDY

Last Name

ALY

MiddleName

A.

Affiliation

Zoology Department, Faculty of Science, Al Azhar University, Assiut, Egypt.

Email

-

City

-

Orcid

-

First Name

AHMED

Last Name

SAYED

MiddleName

B.

Affiliation

Zoology Department, Faculty of Science, Al Azhar University, Cairo, Egypt.

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-

City

-

Orcid

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Volume

23

Article Issue

Issue 2-C

Related Issue

1368

Issue Date

2012-12-01

Receive Date

2012-01-01

Publish Date

2012-12-01

Page Start

61

Page End

72

Print ISSN

1110-2535

Online ISSN

2636-3305

Article File

ABSB_Volume 23_Issue Issue 2-C_Pages 61-72.pdf

PDF . 200.5kB

Link

https://absb.journals.ekb.eg/article_7209.html

Detail API

https://absb.journals.ekb.eg/service?article_code=7209

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Original Article

Type Code

520

Publication Type

Journal

Publication Title

Al-Azhar Bulletin of Science

Publication Link

https://absb.journals.ekb.eg/

MainTitle

VARIATION OF PROTEIN PROFILE AMONG CONSECUTIVE STINGS OF THE SCORPION PARABUTHUS LEIOSOMA (FAMILY: BUTHIDAE) FROM EGYPT, SUPPORTS THE VENOM-METERING HYPOTHESIS IN SCORPIONS.

Details

Type

Article

Created At

22 Jan 2023