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7212

ISOLATION AND CHARACTERIZATION OF NOVEL EXTREMELY HALOTHERMOPHILIC BACTERIUM, HALOMONAS CASEINILYTICA WN.1B.S, FROM WADI AN NATRUN, EGYPT

Article

Last updated: 22 Jan 2023

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Subjects

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Tags

Botany
Microbiology
Zoology

Abstract

The purpose of the present study was to isolate microbial halothermophiles from hyper saline Al- Hamra Lakeat Wadi An Natrun, Egypt. Twenty eight bacterial isolates were obtained and the morphological and physiological properties in addition to enzyme activities were studied. Amongst those isolates, WN.1B.s was selected as the most potent isolate based on growth at high temperature (up to 65oC) and at high salt concentration (up to 34%, near saturation state).A phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate WN.1B.s had the highest sequence similarity with respect to type strains of Halomonas caseinilytica (97 %), Halomonas elongata (96 %), Halomonas eurihalina (95 %), Halomonas koreensis (95 %) and Halomonas halmophila (95 %).Based on physiological characteristics and16S rRNA sequence analysisthis isolate was identified as Halomonas caseinilyticaWN.1B.s which belonged to bacterial domain, class Gammaproteobacteria, order Oceanospirillales, family Halomonadaceae, Halomonas species. Enzyme screening for strain WN.1B.s showed that, the isolate secrete amylases, lipases, cellulases and pectinase enzymes under harsh conditions that may be useful in different industrial processes.

DOI

10.21608/absb.2012.7212

Keywords

HALOTHERMOPHILIC BACTERIUM, HALOMONAS CASEINILYTICA WN.1B.S,, WADI AN NATRUN, Egypt

Authors

First Name

S.

Last Name

EL-LOUBOUDY

MiddleName

S.

Affiliation

Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

EL-GAMAL

MiddleName

S.

Affiliation

Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

H.

Last Name

MAHDY

MiddleName

M.

Affiliation

Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

MOHAMED

MiddleName

G.

Affiliation

Botany and Microbiology department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

23

Article Issue

Issue 2-C

Related Issue

1368

Issue Date

2012-12-01

Receive Date

2012-01-02

Publish Date

2012-12-01

Page Start

33

Page End

48

Print ISSN

1110-2535

Online ISSN

2636-3305

Article File

ABSB_Volume 23_Issue Issue 2-C_Pages 33-48.pdf

PDF . 405.0kB

Link

https://absb.journals.ekb.eg/article_7212.html

Detail API

https://absb.journals.ekb.eg/service?article_code=7212

Order

3

Type

Original Article

Type Code

520

Publication Type

Journal

Publication Title

Al-Azhar Bulletin of Science

Publication Link

https://absb.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023