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A HIGH POTENT ANTIBODY RESPONSE IN RABBITS INFECTED WITH ULTRA VIOLET ATTENUATED CERCARIAE

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Last updated: 03 Jan 2025

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Botany
Microbiology
Zoology

Abstract

Ultraviolet (UV) light is an electromagnetic radiation below the visible wavelengths. Their use in cercariae attenuation needs to be examined. In the present work, Schistosoma mansoni cercariae was exposed to UV radiation. Few studies showed previously an inflicted damage seen on the adult schistosome worms developed from irradiated cercariae. The aim of the study was to find out whether this damage was attributed to direct effect of UV-irradiation on cercariae or due to the host's immunogenicity induced by UV- irradiated cercariae. Single as well as multiple exposures of cercariae to UV Light were examined. They were exposed to UV light, for 1, 2 and 3 hrs and then, were subjected to different treatments: the first one was for the assessment of cercarial viability after one hour of the 3 different treatments. The second one was for rabbit infection and the third one was for cercarial antigen preparation. The cercarial antigens (Ag) were recognized by protective antibodies (IgG1 fractions) which then, were separated and purified from vaccinated rabbit's serum. This cercarial Ag was identified as UVISmC1 gene encoding a protein showing 100% identity at the amino acid level with previously identified S. mansoni clones; theses clones are encoding 51.7 kDa antigens elicited as a result of direct effect of UV radiation on cercariae as well as host's immunogenicity induced by UV irradiated cercariae. This was verified by the recognition of this Ag prepared from E coli clones isolated from cDNA expression library. In conclusion, the data showed a remarkable potency of the UV-radiation-attenuated cercaria in eliciting differential high effectiveness in Ab response under laboratory conditions at 1, 2 and 3 hrs of UV light output at 254 nm. An understanding of the protective immune response elicited by RA cercaria may help in designing a candidate vaccine which is still needed.

DOI

10.21608/absb.2011.7029

Keywords

UV light, Schistosoma mansoni, radiation-attenuated cercariae, purification of IgG and vaccine

Authors

First Name

HALA

Last Name

MOUSTAFA

MiddleName

-

Affiliation

Medical Biophysics Department, Faculty of Applied Medical Science, 6 October University, 6 October, Egypt.

Email

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City

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Orcid

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First Name

SAMAR

Last Name

HASSAB-ALLAH

MiddleName

-

Affiliation

Biochemistry Department, Faculty of Physical Therapy, 6 October University, 6 October City, Egypt

Email

-

City

-

Orcid

-

First Name

HARBI

Last Name

ALI

MiddleName

-

Affiliation

Biology Department, Faculty of Science, Jazan University, Saudi Arabia

Email

-

City

-

Orcid

-

First Name

HANAN

Last Name

RABEE

MiddleName

-

Affiliation

Narcotic Department, National Center for Social and Criminological Research, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

RAGAA

Last Name

ALI

MiddleName

-

Affiliation

Biology Department Basic Science, 6 October University, 6 October City, Egypt.

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City

-

Orcid

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Volume

22

Article Issue

Issue 2-C

Related Issue

1339

Issue Date

2011-12-01

Receive Date

2011-07-03

Publish Date

2011-12-01

Page Start

1

Page End

16

Print ISSN

1110-2535

Online ISSN

2636-3305

Article File

ABSB_Volume 22_Issue Issue 2-C_Pages 1-16.pdf

PDF . 207.0kB

Link

https://absb.journals.ekb.eg/article_7029.html

Detail API

https://absb.journals.ekb.eg/service?article_code=7029

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Original Article

Type Code

520

Publication Type

Journal

Publication Title

Al-Azhar Bulletin of Science

Publication Link

https://absb.journals.ekb.eg/

MainTitle

A HIGH POTENT ANTIBODY RESPONSE IN RABBITS INFECTED WITH ULTRA VIOLET ATTENUATED CERCARIAE

Details

Type

Article

Created At

22 Jan 2023