Ismailia Canal is considered as one of the most important drinking and irrigation water source in Egypt. Twenty four water samples were collected during four successive seasons from   summer 2010 to spring 2011 along the area extending from  El Mazallat to Anshas . Physical parameters temperature, E.C and TDS and chemical parameters  pH ,alkalinity, TH, DO, COD, BOD,  NH₃   ,NO₂^- ,NO₃^- , PO₄^-3 , Cl^-, F^-, Br^- ,SO₄ ^-2 ,Ca²⁺,Mg²⁺, Na⁺, K⁺,  Fe⁺³,  Cd⁺², Zn⁺², Cu⁺², Pb⁺², and  Al⁺³  were analyzed to identify water quality of Ismailia Canal. The results of these analyses, when compared with Egyptian and international standards were found to be within the permissible   limits except COD, NH_3, Fe⁺³, Cd⁺², Pb⁺², and  Al⁺³  at certain sites and/ or certain seasons. Additionally, the naturally occurring radioactive materials (²³⁸U, ²³²Th and ⁴⁰K) were analyzed where ²³⁸U and  ²³²Th were found to exceed EPA limit.  Finally water quality index (WQI) was calculated to be 80.6 and 84.3 according to Egyptian higher committee of water and WHO   guidelines,  respectively; this indicates that the water quality of Ismailia Canal was good.