A sensitive, precise, and accurate gas chromatography-mass spectrometry method was developed for the simultaneous determination of levamisole and oxyclozanide in pure samples and pharmaceutical preparation. Gas chromatography was presented as a simple separation analytical method for the simultaneous analysis of the deliberated drugs within a shorter analytical run time. In this study, separation was achieved using a ZB5 column with (30 m ×0.53 mm, 1.50 µm), and helium as a carrier gas. The proposed method showed well separation between the drugs and each other and had good accuracy. The method showed to be linear (r² = 0.9998), precise (RSD < 0.496%), accurate (recovery of 99.37% for levamisole and 100.14% for oxyclozanide), specific and robust. LOD and LOQ values were 0.935 ng mL^-1 and 3.085 ng mL^-1 respectively for levamisole and 0.884 ng mL^-1 and 2.917 ng mL^-1 respectively for oxyclozanide. The proposed method obtained well separation and had perfect accuracy. The method was validated according to ICH guidelines and carried out to determine the cited drugs in their pharmaceutical preparation.