Capparis cartilaginea is an important medicinal and industrial plant with various bioactivities compound, C. cartilaginea which is difficult to propagate in normal conditions. For establishment and multiplication, MS mediumtreated with crude extract of Spirulina platensis as a source of phytohormones was achieved. Growth parameters of calli revealed that the greatest shoot length (4.08 ± 0.86) and leaf number per shootlet (8.40 ± 1.14) on MS medium supplemented with 4mg/L of algal extract. The phenolic content was measured by the Folin–Ciocalteau method. Results of this study showed that application of crude extract Spirulina platensis at 4mg/L significantly increased the content of phenolic compounds in C. cartilaginea (1.01 mg/g dry mass).HPLC analysis of phytohormones of methanol extract of S. platensis recorded the contents of gibberellins, kinetin and adenine as 241.6, 150.2 and 140.6 ppm respectively.