Background: urinary tract infections is one of the most prevalent bacterial infections worldwide. The present study aims to study and survey different isolates from urine specimens from different countries to assess their prevalence and their relation to urinary tract infections. One hundred and sixty-eight samples were randomly collected from three countries, Egypt, Sudan and Saudi Arabia, as follows: 43, 33 and 92, respectively. Samples were collected from private laboratories and from both sexes. The ages of the patients differed between three years and 89 years for females and from one year to 85 years for males between 4 / 2015-7 / 2016. All samples were inoculated on different selective and differential sterile culture media. After growth, isolated bacteria were identified by physiological and biochemical characteristic. Among all clinical samples, five bacterial genera were detected. The isolates were identified as Escherichia coli (93), Klebseilla spp < /strong>. (32), Pseudomonas spp < /strong>. (26), Proteus spp < /strong>. (14), and Staphylococcus spp < /strong>. (3). Regarding the sex of the patient, this study showed that females are more likely to be infected than males, with 53 (31.55%) of the patients being males and 115 (68.45%) of being females. The most common UTI bacterium was Escherichia coli, followed by Klebseilla spp < /strong>. Majority of female infected cases were in menstruation age stage (14-44 years), while majority males were in old age stage (52-85 years). Surveys and studies of infectious factors are considered one of the most important epidemiological tools for tracking infectious diseases and predicting disease patterns, especially with regard to urinary tract infections due to their widespread prevalence and serious complications between the sexes at the global level.