Cypermethrin is a widespread pyrethroid pesticide and is an environmental pollutant because of its toxicity and persistence. Biodegradation of such chemicals by microorganisms may provide a cost-effective method for their detoxification. Sixty-two cypermethrin degrading bacteria were isolated from Egyptian contaminated soil and water samples. The most potent bacterial strain was identified as Ochrobactrum intermedium SP9 based on 16S rRNA gene sequence analysis, as well as several morphological, physiological and biochemical characteristics examinations on classical level. The maximum biodegradation of cypermethrin by bacterial strain SP9 was achieved at 35°C and pH 7.0, and the degradation rate reached 69.1% within 8 days under the optimal conditions. Hence, the Ochrobactrum intermedium SP9could potentially be used in the future for bioremediation of cypermethrin contaminated soil.