A field experiment was conducted during two successive seasons of  2014/2015 and 2015/2016 at Phytochemistry Department  and Farm of Applied Research Center of Medicinal Plants (ARCMP) affiliated to the National Organization for Drug Control And Research (NODCAR). Present work aimed to study the effect of inoculation Apium graveolens L. seeds with arbuscular mycorrhizal fungi (my) and/or microbein (mi) and/or foliar spray plants with thidiazuran (TDZ)  combine with chemical fertilizer at half or full dose of NPK on  the % of the volatile oil of celery seeds, physiochemical investigation (Specific gravity of each essential oil and Refractive index of each essential oil), Compositional analysis by GLC of essential oil content , total phenolic t and flavonoid contentof dry seeds. Were estimated the results showed that inoculation of celery (Apium graveolens L.) seeds with mixture of mycorrhizal and microbein at full dose of NPK gave the highest yield of the volatile oiland total phenolic andflavonoid contentof dry seeds. While treated plants with biofertilizer (mycorrhizal and/or microbein) /or sprayed plants with (TDZ) combine biofertilizer at half or full dose of NPK didn't have any significant effect on physiochemical investigation and compositional analysis of volatile oil by GLC  as compared to the control.