Chronic liver diseases one of the most common causes of death in the world due to different etiologies. Carbon tetrachloride (CCl₄) one of the well known toxicant used in most of acute and chronic liver injury. Therefore, several researches have been directed toward natural products to develop a new pharmacologically effective agents in the treatment of liver diseases. Matthila arabica Boiss.  one of the brassicaceaeʼs plants which is famous with the presence of pharmacologically active glucsinolates hydrolysis products (GLCsHPs).  The present study was performed to investigate the hepatoprotective effect of (GLCsHPs) fraction of M. arabica against  carbon tetrachloride (CCl₄) induced acute liver injury  at a dose of 1ml/kg injected intraperitoneally as a mixture with corn oil in the seventh day. The (GLCsHPs) fraction was administered in different three doses of 7.5,15 and 30mg/kg orally for six consecutive days followed by single dose of CCl₄ at a dose of 1ml/kg injected intraperitoneally as a mixture with corn oil in the seventh day. The hepatoprotective effect was assessed via liver function tests and histopathological liver sections. Significant reduction in the hepatic enzymes levels was found in animals treated with different doses of (GLCsHPs) fraction particularly at a dose of 30mg/kg as well as restoration of hepatocellular architecture. These findings is indicative for the promising hepatoprotective effect of (GLCsHPs) fraction against CCl₄-induced acute liver injury.