Tremendous concerns have been raised about the possibility that exposure to electromagnetic radiation (EMR) from mobile phones could affect people's health.  Many experimental studies suggest the possible role of reactive oxygen species (ROS) in EMR-induced damage in brain tissues with subsequent neurobehavioral changes. The aim of the present study was to investigate the protective effect of thymoquinone (TQ) on EMR-induced oxidative damage in brain tissue of rats. Results of the current study show that exposure to EMR decreases the time spent in target quadrant of Morris water maze and the percentage time spent in open arms of elevated plus maze, and increases significantly the percentage time spent in closed arms of elevated plus maze, malondialdehyde (MDA), nitric oxide (NO), and norepinephrine (NE) contents in brain tissue. Furthermore, a significant decrease in brain catalase (CAT) activity, reduced glutathione (GSH) contents, serotonin (5-HT) and dopamine (DA) contents were also observed. These alterations were prevented by TQ (5, 10 mg/kg). The observed neuroprotective effects of TQ were attributed to its powerful antioxidant activities.