This study was conducted duringtwo successive seasons of 2012-2013 and 2013-2014 at Biotechnology Department, Phytochemistry Department and Farm of Applied Research Center of Medicinal Plants (ARCMP) affiliated to the National Organization for Drug Control And Research (NODCAR). The present work aimed to investigate the effect of inoculation Pimpinella anisum L. seeds with arbuscular mycorrhizal fungi (my) and/or microbein (mi) biofertilizer and foliar spray plants with Thidiazuron (TDZ)  combined with chemical fertilizer at half or full dose of NPK on number of spores Am fungi (kg soil^-1), AM fungi colonization , enzymatic activities  (dehydrogenase activity [µg TPF/g dry soil/day] & Nitrogenase activity [nmol C₂H₄/g rhizosphere/ hour]), growth parameters (fresh weight of shoots per plant (g), fresh weight of roots per plant (g), dry weight of shoots per plant (g), dry weight of roots per plant (g), Plant height (cm), number of umbel per plant [at full flowering stage] & dry weight of seeds per plant [at harvest stage]) and chemical composition (plant pigments [chlorophyll a, chlorophyll b and carotenoides], macro elements content (%), total carbohydrates, and crude protein). The results in both seasons showed that, the highest values of number of AM fungi spores (kg soil^-1) in anise (Pimpinella anisum L.) roots, AM fungi colonization %, enzymatic activities, growth parametersand chemical composition obtained at inoculating seeds with mixture of mycorrhizal and mycrobein at full dose of NPK.